Tools now exist that enable the extraction of data from images and video for a wide-range of biological systems. I use these approaches to implement high-throughput methods that often yield higher-quality data, new types of data, and substantially more data than previous efforts. I will discuss three examples connected to ongoing research in my lab. First, I will describe new software for automatically measuring vessel dimensions and geometry from three-dimensional angiographic (e.g., CT and MRI) images. Because these measurements are non-invasive, we can avoid concerns about distortion or destruction of the vasculature, thus leading to more reliable measurements. Second, I will show how video-tracking software is being used to track wingless fruit flies that are being hunted by wolf spiders. These high-resolution measurements allow us to study components of consumer-resource interactions that have rarely been measured before. Finally, I will outline new software to identify individual bacterial colonies grown in agar plates, measure their sizes, and construct size distributions. With these measurements, we can analyze how the phenotype of colony size, including coefficient of variation and other measures of the size distribution, responds to a range of concentrations of antibiotics, potentially helping to reveal how bacterial diversity relates to the evolution of resistance.